The school is one of the places where various techniques and procedures are explicitly produced and practice in order for the students to attain learnings and become competent in many aspects in life. This study aimed to assess and determine the ethno-learning resources in teaching biology. Also, the study aimed to answer the following questions: What are the ethno-learning resources used in teaching Biology? What are the issues and problems encountered in using the instructional materials of the teacher? What are the most effective ethno-learning resources in teaching Biology? What instructional materials can you recommend to develop in teaching Biology? This was conducted from selected schools in Butuan City East Districts I, II, & III, particularly in Ampayon National High School, Taligaman National High School, Basag National High School, Los Angeles National High School, and Anticala National High School. There were 31 sciences teachers from selected schools in junior high school in Butuan City East Districts I, II, & III who were taken as participants of the study. The findings indicate books, laboratory apparatuses, modules, worksheets, and laptop are more available in their school. Furthermore, books and real pictures are more effective in teaching biology. This revealed that the science teachers are more resourceful in terms of collecting possible instructional learning resources in a community. It was concluded that the instructional learning resources such as real objects and pictures, indigenous materials and books were plainly effective in the field of teaching Biology. This leads the researchers to design an Instructional Material including real pictures in the community for better understanding of the learning competencies in Biology.